• 商品貨號： FH1597

• 規格：10μl質粒
• 啟動子：U6 promoter
• 復制子：pUC ori
• 終止子：SV40 poly(A) signal
• 質粒大?。?/span>5141bp
• 原核抗性：氨芐青霉素Amp
• 篩選標記：綠色熒光ZsGreen1
• 克隆菌株：大腸桿菌Stbl3
• 質粒簡介：             pDC316-ZsGreen1-shRNA質粒是一個Admax腺病毒RNA干擾載體，可通過Pst1和BamHI酶切位點連入shRNA片段，并由U6啟動子驅動。
• 質粒圖譜1： png/1-1G026152011I2.png

質粒序列： 點擊查看序列

LOCUS       Exported                5141 bp ds-DNA     circular SYN 26-OCT-2017

DEFINITION  synthetic circular DNA

KEYWORDS    pDC316-ZsGreen1-shRNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5141)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.miaolingbio.com

FEATURES             Location/Qualifiers

     source          1..5141

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    469..992

                     /label=MCMV promoter

     CDS             1003..1698

                     /codon_start=1

                     /product="Zoanthus green fluorescent protein"

                     /label=ZsGreen1

                     /note="mammalian codon-optimized"

                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN

                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV

                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG

                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH

                     AIASGSALP"

     polyA_signal    1836..1957

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     promoter        1975..2215

                     /label=U6 promoter

                     /note="RNA polymerase III promoter for human U6 snRNA"

     polyA_signal    2264..2398

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     protein_bind    2418..2451

                     /label=loxP

                     /bound_moiety="Cre recombinase"

                     /note="Cre-mediated recombination occurs in the 8-bp core 

                     sequence (GCATACAT)."

     rep_origin      complement(2875..3463)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3634..4494)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4495..4599)

                     /gene="bla"

                     /label=AmpR promoter

     misc_feature    4682..453

                     /label=Ad

ORIGIN

        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt

       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg

      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg

      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt

      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa

      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg

      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc

      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg

      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc

      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa

      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa

      661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc

      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat

      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg

      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct

      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca

      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcca ccatggccca gtccaagcac

     1021 ggcctgacca aggagatgac catgaagtac cgcatggagg gctgcgtgga cggccacaag

     1081 ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcaggc catcaacctg

     1141 tgcgtggtgg agggcggccc cttgcccttc gccgaggaca tcttgtccgc cgccttcatg

     1201 tacggcaacc gcgtgttcac cgagtacccc caggacatcg tcgactactt caagaactcc

     1261 tgccccgccg gctacacctg ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc

     1321 tgcaacgccg acatcaccgt gagcgtggag gagaactgca tgtaccacga gtccaagttc

     1381 tacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccga caactgggag

     1441 ccctcctgcg agaagatcat ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc

     1501 atgtacctgc tgctgaagga cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag

     1561 gccaagtccg tgccccgcaa gatgcccgac tggcacttca tccagcacaa gctgacccgc

     1621 gaggaccgca gcgacgccaa gaaccagaag tggcacctga ccgagcacgc catcgcctcc

     1681 ggctccgcct tgccctaaag atctcgagat ccaccggatc tagataactg atcataatca

     1741 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga

     1801 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg

     1861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt

     1921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtctgggca ggaagagggc

     1981 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt

     2041 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa

     2101 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt

     2161 accgtaactt gaaagtattt cgatttcttg ggtttatata tcttgtggaa aggacgaaac

     2221 acctgcagtt ttttggatcc caatgtcgac ctcgacttcg agcaacttgt ttattgcagc

     2281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc

     2341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgt

     2401 ctagcatcga agatccaata acttcgtata gcatacatta tacgaagtta taagtagctt

     2461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca

     2521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact

     2581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct

     2641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc

     2701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca

     2761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg

     2821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca

     2881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa

     2941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc

     3001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc

     3061 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct

     3121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg

     3181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag

     3241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta

     3301 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg

     3361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt

     3421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt

     3481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag

     3541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat

     3601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc

     3661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat

     3721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc

     3781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag

     3841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag

     3901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt

     3961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg

     4021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt

     4081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc

     4141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc

     4201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa

     4261 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg

     4321 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc

     4381 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag

     4441 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt

     4501 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt

     4561 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc

     4621 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac

     4681 tctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcac agcggcagcc

     4741 taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc

     4801 accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac

     4861 taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct

     4921 acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc

     4981 cacgttacgt aacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc

     5041 gccctaaaac ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac

     5101 cccctcatta tcatattggc ttcaatccaa aataaggtat a

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